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KMID : 0604220060130010031
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Korean Journal Investigative Dermatology
2006 Volume.13 No. 1 p.31 ~ p.34
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The Effect of Nicotine on Melanization in Cultured Normal Human Melanocytes
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Choi Chun-Pill
Kim Young-Il
Lee Jin-Woo
Lee Mu-Hyoung
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Abstract
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Nicotine, the active ingredient of tobacco, causes increased platelet aggregation, endothelial cell damage and alterations in prostacyclin and thromboxane metabolism. Skin changes with nicotine include brown pigmentary staining of hands and nails, leg ulcers, cyanosis, decreased skin temperature and other signs of arterial insufficiency. However, the effect of nicotine on melanization is not well established. The purpose of this study is to evaluate the effect of nicotine on melanization in cultured normal human melanocytes. In order to evaluate non-cytotoxic dose of nicotine, we exposed various concentrations(0.01, 0.1, 1, 10, 100, and 1000㎍/ml) of nicotine to melanocytes and examined cell cytotoxicity using MTT assay. There was no cytotoxicity below 0.1㎍/ml of nicotine. After exposure to 0.1㎍/ml nicotine on cultureed normal human melanocytes, we investigated the effects of nicotine on the expression of tyrosinase(TYR), tyrosinase-related protein 1(TYRP1), DOPAchrome tautomerase(DCT) at the protein levels in human skin melanocytes. There was no significant difference in the expression of TYR, TYRP1, and DCT protein after treatment with nicotine compared to the untreated control. These result suggest that short-term nicotine exposure had no direct melanogenic effect, in vitro
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KEYWORD
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Nicotine, TYR, TYRP1, DCT
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